Project 3: Design and Development of Biocompatible Magnetic Nanoparticles With Biomedical Application. (FA2, BB1, CI8)
Description and objectives:
The objectives being pursued are:
1. Synthesis (via coprecipitation in aqueous medium and/or thermal decomposition), and structural and magnetic characterization of iron oxide nanoparticles and cobalt ferrite (silicon oxide coated).
2. Functionalization of magnetic nanoparticles, binding biomolecules such as DNA or proteins, to modify their level of biotoxicity or biocompatibility.
3. Determination of the biocompatibility of these nanoparticles by cytotoxicity assays on different human tumor and non-tumor cell lines.
4. Analysis and quantification of the binding of DNA and proteins to nanoparticles.
Among the applications of new biocompatible magnetic nanoparticles may include medical diagnostics, drug delivery and cancer therapy, although this will require, after the completion of this project, combine nanoparticles with other biomolecules that ensures selectivity in the recognition of target cells.
It's been undergone the study of biocompatibility of ferric nanoparticles (Fe3O4 and CoFe2O4) by analyzing their cytotoxicity on the human tumor cell line, Caco-2.
The first data obtained have led to discard the option to insulate nanoparticles by silica-based coating, which represents an dvance in the possibilities of these nanoparticles in biomedical applications by controlled heating (hyperthermia).
The results were presented in X International Conference on Nanostructured Materials, in Rome; and XII International Biennial Meeting of Thermodynamics Specialist Group (TSG), in Las Palmas de Gran Canaria