Scientific Infrastructure Platforms.
1. Proteomics and Genomics Platform
This platform, that is integrated into the Proteored subnode of Northwest Node (Genome Spain), has the following equipment:
- Ion cyclotron resonance Mass Spectrometer (FTICR-MS) with MALDI, Electrospray (ESI) and APCI sources of ions, for Proteomics / Genomics and Structural Biology, with resolution> 100,000 and high resolution accuracy of> 1 ppm.
- MALDI-TOF (Bruker Microflex)
- Autoflex III TOF / TOF (Bruker)
- SPII Proteine gels chopper
- Digester gels Proteine Bruker DP
- Nano-HPLC Dionex for sample preparation and injection.
- X-ray diffractometer
With the addition in 2009 of massive genomic analysis team will produce a platform for genetic analysis and functional genomics with a wide range of applications including genome sequencing of complete and candidate regions, expression profiling, identification and quantification of sRNA . It has the potential to generate more than 100,000 million database for each transaction, generatind global genome expression profiling of any transcript, any gene in any species without having to know in advance of its transcriptome.
- ABI Prism DNA sequencer.
- Mass Genomic Analysis System
2. Bioinformatics Platform
Response to demanding molecular medicine and personalized healthcare will require the development of a support unit for medical Bioinformatics. Its functions will manage and analyze complex, multilevel data, ranging from the genotype to phenotype, and environmental factors. It will contribute to understand the causes of disease and advance the development of diagnostic and therapeutic systems more efficient, safer and adapted to the peculiarities of patients.
This platform is to manage repositories of molecular information on genetic causes of diseases, records on the characteristics and genetic differences among patients, personal data health and medical history virtual medical information sources of interest as well as databases containing information on diseases for the practice, clinical trials and global health knowledge bases disaggregated levels-regional, national or international, with population data, related epidemiological and environmental factors, health indicators, etc..
Goals to be achieved are:
- Development of an integrated system for accessing objects, tools and databases of genotypes and phenotypes.
- widespread adoption of technology "semantic web" (as XML) for exchanging massive data.
- Integration of the series of collaborative work tools, from access to Web conferencing systems to integration in "e-science" software groups and representations of biological objects.
- The development of a public section, to chanel communications.
3. Image Platform.
It aims to support researchers at the Center in the following areas:
- Use of metal nanoparticles as markers to facilitate identification of components in tissue through imaging microscopy.
- Synthesis of semiconductor nanoparticles (quantum dots) for cell labeling and fluorescence imaging in the near IR.
- Analysis of tissues and cells and semiconductor nanoparticles labeled with other fluorescent markers.
- Analysis of tissues and cells labeled with metal nanoparticles (methods based on light scattering by surface plasmons and SERS).
- Observation of cellular systems and tissues by electron microscopy and confocal microscopy.
- The distribution maps obtained by staining tissues with nanoparticles designed to recognition by SERS.
- The study of the use of metal nanoparticles as contrast agents in ultrasound.
- SEM-FIB dual microscope
- System for the analysis of gels and membranes of DNA, RNA and proteins by fluorescence Ettan DIGE Imager and Typhoon Trio autoradiography
- image analysis system ANALYZER1000 IN CELL
- microscope slices of the nervous system and IR camera and control system of T
- EMC BIORAD Confocal Microscope coupled to Nikon Eclipse 800 microscope
- Confocal Raman Microscope Jovin-Yvon
- Beckman Coulter flow cytometer
- Flow cytometer Flowcam
- Secondary Ion Mass Spectrometer (TOF / SIMMS-IV)
- Scanning probe microscope (SPM) Veeco NanoScope IV
- Scanning Near Field Optical Microscope (SNOM) Veeco Aurora 3
- Veeco Nanoindenter
- Profilometer Veeco NT 1100
4. Preclinical Testing Platform and Tools for Diagnosis and Treatment
Researchers will be serviced through centralized facilities:
- cell cultures
- monoclonal antibodies and phage libraries
- nanoparticles for diagnosis and therapy
For studies in vivo, it will be used the laboratories and operating rooms of the animal house, which is annexed, in the Biomedical Module of CITEXVI Project, which will have stat of the art facilities for supply and breeding of experimental animals, test laboratories, transgenic animals lab and a special lab (P2 grade) for TSE testing (Transmissible spongiform encephalopathies).
5. Platform HTScreening
It offers services to researchers:
- The discovery of small molecules that act as modulators of biological functions.
- The management of compound libraries generated by synthesis from natural sources or purchased commercially.
- The development of display technology and image of cells and tissues using molecules.
- liquid handling robots, 96 or 384 wells.
- multimode plate readers with the possibility of kinetic analysis.
- automated microscopy system.
- Nano-Plotter NP System ™ 2 GeSiM
- System analysis of interactions between biomolecules Biacore T100; biosensor Surface Plasmon Resonance (SPR)
6. Platform for Bio-statistics
Unique platform to share the following objectives:
- Development of a Statistical Consulting Laboratory for professionals-including SERGAS biosanitary and businesses-and for multi-and interdisciplinary interaction of groups
- Comprehensive development of biometric tools for biomedical data analysis of a complex nature: motivation, formal definition, checking theoretical studies via simulations, implemented in a programming language, friendly biostatistician software development
- Conducting user training monographic courses for professionals and researchers related to the biosanitary sector on statistical techniques of increasing interest in biomedicine, including training and handling of commercial statistical software and free software
- Development of enormous biomedical statistics on comprehensive databases in oncology, epidemiology, neurology, or genetics
- Organization of seminars, visits and short stays of internationally recognized biostatisticians of biomedical expertise, and pursuing collaborations.
- Organization of " modeling weeks in biostatistics ", that are intensive meetings lasting 4-5 days over which to study and propose a bio-statistical solution to a problem raised by a company or group of biosanitary practitioners