We aim at creating a stable and quality driven research cluster in Biomedicine.

Research Frame.

AREA 3: NEW TOOLS FOR PREVENTION AND TREATMENT .

3A. Antibodies and drugs conjugated to nanoparticles.
 Groups: QF1, IN1, QO1, FB3, CI8.

 OBJECTIVES:
- Development of new antibodies for use in human therapy. Studies of response of macrophages and tumor cells in the presence of several types of nanoparticles to determine the intrinsic toxic effects of nanoparticles and their immune response.
- Development of nanoparticles for human vaccination and therapy. Conjugation of antitumor drugs (epi-drugs, kinase inhibitors) to nanoparticles for cancer chemotherapy.
- Detection of nanoparticles by conventional ultrasound methods and use as ultrasound contrast enhancement in different in vivo tissues. The ultrasound diagnostic techniques are routine in the clinic for its high diagnostic capability, easy implementation and low cost. However, to date it has not been achieved specific contrast media that enables enhanced display of specific tissues, as with the other imaging techniques. Functionalized gold nanoparticles can be detected in vitro and in vivo in lab animals by conventional methods configured for routine ultrasound, according to previous studies of our group. It is aimed to develop means of tissue-specific enhanced display because the sonographic brightness of nanoparticles depends on their size, shape and concentration in the tissue.

3B. New drugs.
 Groups: QO1, QF1, IN1, BB1, CI8, FB3, EQ2, EQ9.

 OBJECTIVES:
- Solid-phase synthesis and drug discovery over SiO2 microbeads on nanoparticles. Fabrication and characterization of composites of silver / gold nanoparticles and SiO2 microbeads (transparent to visible light) and its employment in Biomedicine. After the self-assembly of a short chain thiol functionalized on the surface of the nanoparticle, it can be used as a matrix for the peptide synthesis in solid phase and subsequent ultrafast detection using Raman spectroscopy, SERS and SERRS.
- Design and synthesis of novel epi-drugs based on natural products, isolation and identification of epigenetic enzymes and other receptor proteins by affinity chromatography, and also, development, optimization and application of isotopic labeling techniques such as SILAC and Itraq. Study of new epi-drugs for culture neurons behavior and application in the treatment of CNS diseases such as Alzheimer's, Huntington's and ALS. Effect of the combination of histone deacetylase inhibitors (HDACs, such as sodium valproate) and inhibitors of histone methyl transferases (KMTS) and DNA methyl transferases (DNMTs) in neurodegeneration.
- Drug Design against therapeutic targets for colorectal cancer identified in proteomic studies of differential expression.
- Study the effect of new ligands of RXR and PPAR receptors in metabolic diseases. Mechanism and information integration on metabolic pathways. RXR and PPAR receptors are transcription factors ligand-dependant. There are several drugs useful in treating type II diabetes and obesity that act by modulating PPAR activity (glitazones), though of limited use for unwanted side effects. The development of new ligands that avoid these effects would allow its immediate applicability to the treatment of most common metabolic disease around, the DM-II.
- Purification of plant extracts to produce oligosaccharide and oligourónidos, its structure determination, and evaluation of biological activities (effects on intestinal flora, immune system, lipid and carbohydrate metabolism). Study of immunostimulatory properties of oligosaccharides and oligourónidos derived from biomass, and the mechanisms of immunostimulation. Following tests for quantitative assessment of immunostimulant properties.

3C. Natural antioxidants.
 Groups: EQ2, EQ9, QO1, IN1, CI8.

 OBJECTIVES:
- Natural antioxidants (carotenoids and antioxidant dietary biomass) and therapeutic applications. Isolation and structural determination of biologically active compounds derived from biomass. Structure-function interrelationship in the biological properties of natural antioxidants.
- In vitro evaluation of antioxidant activities of molecules isolated from biomass, through chemical techniques and cell culture based. Implementation of new analytical techniques for determining the composition of biomass hydrolysates and rapid methods for quality control of products derived from biomass.
- Effects of antioxidants in preventing cancer and cardiovascular disease.

3D. Thermotherapy.
 Groups: FA2, QF1.

 OBJECTIVES:
- New materials for thermotherapeutic applications: synthesis, characterization and applications.
- Nanostructured peloids thermotherapeutic agents: synthesis, thermophysics characterization and applications in spas related to thermodynamic aspects.
- Magnetic nanoparticles thermotherapeutic agents: synthesis and characterization of magnetic composites involving optimization of parameters related to physical phenomena involved in the hyperthermia and the use of contrast agents.